Cryo-EM Services

We've already industrialized protein crystallography, now we're industrializing cryo-EM. We strive to deliver elucidation of even the most complex structures through the establishment of the RIGHT protein and application of premium cryo-EM platform technology and expertise.

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Proteins, Structures and Assays

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Access to our world-class protein science

With our industrial cryo-EM Services, you get full access to our world-class protein science, feasibility analysis and structural determination helping you to Reach Right Faster.

Our cryo-EM Services is sub-divided into three major sections. You can make use of the recommended full service or pick and choose individual service elements according to your project demand!

 
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PROTEIN SCIENCES

  • Construct design
  • Expression systems: E.coli, insect, mammalian, yeast
  • Purification and rigorous analytics-driven Quality Control
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EM FEASIBILITY ANALYSIS

  • Purity, Homogeneity and Stability testing
  • Negative Staining EM
  • Ligand-Protein interaction analysis
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STRUCTURE DETERMINATION

  • Cryo-EM condition optimization
  • Low-Resolution cryo-EM and/or
  • High-Resolution cryo-EM

SERVICE PROCESS

Protein for cryo-EM

  • Construct design
  • Gene synthesis
  • Expression
  • Purification
  • Analytics-driven Quality Control
  • Product and data delivery

EM feasibility Analysis

  • Negative staining EM
  • 2D classification
  • EM feasibility (to prove homogeneity and complex stability)
  • Data driven decision on "how-to-proceed"
  • Optimization of cryo-EM grid freezing conditions and Cryo-EM 2D classification

 

 

First Structure

  • Collection of 12 h data sets (Glacios)
  • Data processing and structure refinement
  • Low-resolution cryo-EM structure (3D envelope, < 10 Å)
  • X-ray structures can be fitted into density
  • Visualization of protein/complex architecture
  • Visualization of antibody-antigen interaction

High-resolution cryo-EM

  • Collection of 24 - 72 h datasets (Glacios & Krios)
  • Data processing and structure refinement
  • High-resolution cryo-EM structure (visible side-chains, < 4 Å)
  • Visualization of ligand-binding mode
  • Validation of binding mode
  • Enables structure-based drug design

Proteros kick-off cryo-EM Stats

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ongoing cryo-EM projects

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high-resolution cryo-EM structures obtained

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multi-protein enzyme complexes solved at <3.5Å